New publication: Hernandez-Candia, C,N., Brady, B.R., Harrison, E, and Tucker, C.L. A platform to induce and mature biomolecular condensates using light and chemicals. (2024) Nature Chemical Biology. doi10.1038/s41589-023-01520-1.

2020

Hernandez-Candia, C. N. and Tucker, C.L. Optogenetic control of gene expression using cryptochrome 2 and a light-activated degron. Methods in Molecular Biology, doi: 10.1007/978-1-0716-0755-8_10.

Cleveland, J. and Tucker, C.L. Photo-SNAP-tag, a light-regulated chemical labeling system. (2020) ACS Chemical Biology 15, 2212-2220.

Pearce, S. and Tucker, C.L. Dual Systems for Enhancing Control of Protein Activity through Induced Dimerization Approaches, Adv. Biology. doi.org/10.1002/adbi.202000234

2019

Meador, K.M., Wysoczynski, C.L. Norris, A.J., Aoto, J., Bruchas, M.R., and *Tucker, C.L. Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent. (2019) Nucleic Acids Res. gkz585 https://doi.org/10.1093/nar/gkz585

^Liu, Q., ^Sinnen, B.L., Boxer, E.E., Buchta, W.C., Schneider, M.W., Gibson, E.S., Wysoczynski, C.L., Gottschalk, A., Aoto, J., *Tucker, C.L., and *Kennedy, M.J. A photoactivatable botulinum neurotoxin for inducible control of neurotransmission. (2019) Neuron 101, 863-875.
^Co-first author *Co-corresponding author. (link to article)

Spiltoir, J.I. and Tucker, C.L. Photodimerization systems for regulating protein-protein interactions with light. (2019) Curr. Opin. Struct. Biol. 57, 1-8. (link to article)

Hernandez-Candia, C. N., Wysoczynski, C.L., and Tucker, C.L., Advances in optogenetic regulation of mammalian cells using cryptochrome 2 (CRY2). (2019) Methods, doi.org/10.1016/j.ymeth.2019.03.011 (link to article)

2018

Cook, S.G., Bourke, A.M., O'Leary, H., Zaegel, V., Lasda, E., Minze-Berge, J., Quillinan, N., Tucker, C.L., Coultrap, S.J., Herson, P.S., and Bayer, K.U. Analysis of the CaMKIIalpha and beta splice-variant distribution among brain regions reveals isoform-specific differences in holoenzyme formation. (2018) Scientific Reports 8, 5448. (link to article)

Locke, C., Machida, K., Tucker, C.L., Wu, Y., and Yu, J. Optogenetic activation of EphB2 receptor in dendrites induced actin polymerization by activating Arg kinase. (2017) Biol. Open 6, 1820-1830.

2017

Pathak, G., Spiltoir, J.I., Hoglund, C., Polstein, L.R., Heine-Koskinen, S., Gersbach, C.A., Rossi, J., and Tucker, C.L. (2017) Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res. doi 10.1093/nar/gkx260. (link to article)

Liu, Q., and Tucker, C.L. Engineering genetically-encoded tools for optogenetic control of protein activity. (2017) Current Opinion in Chem. Biol.40, 17-23. (link to article)

2016

Taslimi, A., Zoltowski, B., Miranda, J.G., Pathak, G., Hughes, R.M., and Tucker, C.L. (2016) Optimized second generation CRY2/CIB dimerizers and photoactivatable Cre recombinase. Nat. Chem. Biol. 12, 425-430. (link to article) (view-only PDF)

Spiltoir, J.I., Strickland, D., Glotzer, M., and Tucker, C.L. (2016) Optical control of peroxisomal trafficking. ACS Synthetic Biology 5, 554-560.(Link to article)

2015

Schindler, SE., McCall, J.G., Yan, P., Hyrc, KL., Li, M., Tucker, C.L., Lee, J.M., Bruchas, M.R., Diamond, M.I. (2015) Sci. Reports 5, 13627. (link to article)

2014

Pathak, G.P., Strickland, D., Vrana, J.D., and Tucker, C.L. (2014) Benchmarking of optical dimerizer systems. ACS Synthetic Biology, 3, 832–838. (link to article)

Taslimi, A., Vrana, J.D., Chen, D., Borinskaya, S., Mayer, B., Kennedy, M.J., and Tucker, C.L. An optimized optogenetic clustering tool for probing protein interaction and function. (2014) Nature Commun. 5: 4925. **Highlighted in Nature Methods, Nov 2014 issue** (link to article)

Tucker, C.L., Vrana, J.D., and Kennedy, M.J. (2014) Tools for controlling protein interactions with light. Curr. Protoc. Cell Biol. 64, 17.16.1-17.16.20.

Hansen, M.G., Fregoso, V., Vrana, J.D., Tucker, C.L., and Niswander, L.A. Peripheral nervous system defects in a mouse model for peroxisomal biogenesis disorders. (2014) Dev. Biol.395, 84-95

Lage, M.D., Pittman, A.M., Roncador, A., Cellini, B., and Tucker, C.L. (2014) Allele-specific characterization of alanine:glyoxylate aminotransferase variants associated with primary hyperoxaluria. PLOS ONE 9, e94338.

2013

Pathak, G.P., Vrana, J.D., and Tucker, C.L. (2013) Optogenetic control of cell function using engineered photoreceptors. Biol Cell 105, 59-72.

2012

Pittman, A.M., Lage, M.D., Poltoratsky, V., Vrana, J., Paiardini, A., Roncador, A., Cellini, B., Hughes, R.M., and Tucker, C.L. (2012) Rapid profiling of disease alleles using a tunable reporter of protein misfolding. Genetics 192, 831-842. **Highlighted in Nature Reviews Genetics **

Hughes, R.M., Bolger, S., Tapadia, H., and Tucker, C.L. (2012) Light-mediated control of DNA transcription in yeast. Methods. 58, 385-391.

Hughes, R.M., Vrana, J.D., Song, J., and Tucker, C.L. (2012) A light-dependent, dark-promoted interaction of Arabidopsis cryptochrome 1 and phytochrome B. J. Biol. Chem. 287, 22165-22172. *Paper of the Week*  **Awarded best plant biology paper in JBC in 2012 **

Tucker, C.L. (2012) Manipulating cellular processes using optical control of protein-protein interactions. Prog. Brain Res. 196, 95-117.

Pajerowska-Mukhtar, K.M., Wang, W., Tada, Y., Oka, N., Tucker, C.L., Fonseca, J.P., and Dong, X. (2012) The HSF-like transcription factor TBF1 is a major molecular switch for plant growth-to-defense transition. Curr Biol. 22, 103-112.

2008-2010

Kennedy, M.J., Hughes, R.M, Peteya, L.A., Schwartz, J.W., Ehlers, M.D., and Tucker, C.L. Rapid blue light induction of protein interactions in living cells. (2010) Nature Meth 7, 973-975.

West, G.M., Tucker, C.L., Xu, T., Park, S.K., Han, X., Yates, J.R., and Fitzgerald, M.C. (2010) Quantitative proteomics approach for identifying protein-drug interactions in complex mixtures using protein stability measurements. Proc. Natl. Acad. Sci. U.S.A. 107, 9078-9082.

Hopper, E. D., Pittman, A.M.C., Tucker, C. L., Campa, M. J., Patz, E. F., and Fitzgerald, M. C. (2009) Hydrogen/deuterium exchange- and protease digestion-based screening assay for protein-ligand binding detection. Anal. Chem. 81, 6860-6867.

Tucker, C. L., Peteya, L.. Pittman, M. C., and Zhong, J. (2009) A genetic test for two-hybrid bait competency using RanBPM. Genetics 182, 1377-1379.

Hopper, E. D., Pittman, A.M.C., Fitzgerald, M.C., and Tucker, C. L. (2008) In vivo and in vitro examination of stability of primary hyperoxaluria-associated human alanine:glyoxylate aminotransferase. J. Biol. Chem. 283, 30493-30502.